Tissue Sample Storage Temperature

This article will highlight some of the shortcomings in the use of cold temperature based sample storage describe the new and innovative technologies available today that mitigate these shortcomings and offer suggestions on the convergence of these technologies in meeting the global challenge to be faced as bio specimen collection increases.

Tissue sample storage temperature.

Ambient samples should be protected from heat and moisture. Tissues that can be kept refrigerated above freezing 0 c to 10 c most commonly include placental tissues cellular tissues and osteoarticular or osteochondral grafts. However they can be stored between 20 and 40 c for up to 3 months. Whole tissue bone marrow stem cells sperm etc finding the optimal storage temperature is more complex.

Bone and tendons need to be stored below 40 c for the full shelf life 5 years from donation. Bacteria it can typically be stored at 80 c. Storage we store the tissue in liquid nitrogen tanks and the plasma and serum are stored inside a ultra low temperature freezer at an average temperature of 75 c. The standard weight of our samples is between 0 5 to 1 0 gram.

A cell component dna rna etc it can typically be stored at 20 c 40 c 80 c. This new standard pc 17 10 applies to organizations that store or issue tissue which may include areas outside of. Jcaho temperature standards jcaho tissue standards tissue storage temperatures jcaho standards the joint commission of accreditation of healthcare organizations issued a new standardized procedures on storing tissue samples. Standards have been set for cold storage of tissues which identify acceptable temperature ranges and their impact on shelf life.

Cryo preservation or cryo conservation is a process where organelles cells tissues extracellular matrix organs or any other biological constructs susceptible to damage caused by unregulated chemical kinetics are preserved by cooling to very low temperatures typically 80 c using solid carbon dioxide or 196 c using liquid nitrogen. By reducing sample temperatures to below the glass transition phase of water 132 c all metabolic activity comes to a halt. 2 biosample preservation monitoring and record keeping. The temperature for storage will depend on the tissue.

If your sample is. While many tissue samples are stored in mechanical freezers at 80 c it is important to note that at this temperature metabolic activity has not ceased it has only slowed down. Ultra low freezer storage 80ºc. Dna and rna can typically be obtained from tissues that have been suspended in appropriate solutions before freezing at 20ºc though colder temperatures are recommended for long term storage or for the storage of tissues or cells that are not suspended in a stabilizing solution.